| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TOMER-LAWHEAD, Mira | Blacksburg High School | NT | |
| 2 | SANTIAGO, Natasha | Blacksburg High School | 1:00.27 | East Coast Elite - OMSOC |
| 3 | PROCACCIO, Brooklyn | Christiansburg | 55.63 | All-American Relays |
| 4 | PIETRAVALLE, Olivia | Cave Spring High School | 58.32 | 15th Annual Knights Track Classi |
| 5 | WARREN, Sophia | Blacksburg High School | 1:00.66 | Hidden Valley HS Meet #1 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | O'DELL, Madison | Pulaski County | 54.06 | Region 3D Track & Field Champion |
| 2 | TABOR, Nona | Christiansburg | 52.95 | Terrier Team Challenge |
| 3 | MAHER, Julia | Blacksburg High School | 50.98 | Blue Demon Invitational |
| 4 | TURMAN, Kate | Blacksburg High School | 52.34 | East Coast Elite - OMSOC |
| 5 | COOK, Clara | Blacksburg High School | 53.31 | All-American Relays |
| 6 | PERDUE, Iris | Floyd County | 55.38 | Blue Demon Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GRIGGS, Penny | Blacksburg High School | 49.71 | Cosmopolitan Invitational Track |
| 2 | KING, Olivia | Pulaski County | 49.15 | VHSL Class 3 State Championships |
| 3 | ALTIZER, Leah | Floyd County | 48.81 | VHSL Region 2C Championships |
| 4 | BANDY, Jayla | Blacksburg High School | 48.97 | Blue Demon Invitational |
| 5 | ALDRIDGE, Megan | Floyd County | 49.18 | Three Rivers District Meet |
| 6 | MARRUFO, Catalina | Blacksburg High School | 50.16 | Titan Track Classic |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MARSHALL, Josie | Blacksburg High School | 47.10 | VHSL Class 4 State Championships |
| 2 | LEXIMA, Lindsay | Cave Spring High School | 45.57 | Region 3D Track & Field Champion |
| 3 | GARZA, Mirabella | Blacksburg High School | 44.64 | Golden Eagle Invitational |
| 4 | BALLOWE, Lilly | Blacksburg High School | 45.50 | |
| 5 | DICKHANS, Tess | Blacksburg High School | 47.07 | Blacksburg Invitational |
| 6 | TESI, Sage | Blacksburg High School | 48.20 | Golden Eagle Invitational |