| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HAMMERSLEY, Sophia | Glenvar High School | 49.46 | Blue Demon Invitational |
| 2 | MARSHALL, Josie | Blacksburg High School | 48.50 | VHSL Class 4 State Championships |
| 3 | GARZA, Mirabella | Blacksburg High School | 45.17 | Blue Demon Invitational |
| 4 | BALLARD, Grace | Christiansburg | 48.25 | Region 3D Track & Field Champion |
| 5 | PERRY, Bryanna | Radford High School | 49.24 | Blacksburg Invitational |
| 6 | BOWKER, Tessa | Christian Heritage Academy | 54.87 | Ben Franklin Middle School meet |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BAILEY, Brooklyn | Narrows | 1:00.21 | New River Invitational |
| 2 | GREEN, Kaelynn | Radford High School | 58.14 | VA Middle School Outdoor State C |
| 3 | DEJESUS, Abigail | Blacksburg High School | 55.77 | Blue Demon Invitational |
| 4 | KIRBY, Jackie | Narrows | 57.12 | VHSL 1C Regional Outdoor Champio |
| 5 | FLANDERS, Chelsea | Christian Heritage Academy | 59.80 | Franklin County Home Meet |
| 6 | STOWERS, Justise | Narrows | 1:11.16 | 14th Annual Knights Track Classi |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BANDY, Jayla | Blacksburg High School | NT | |
| 3 | TABOR, Nona | Christiansburg | NT | |
| 4 | REED, Karsyn | Narrows | NT | |
| 5 | PROCACCIO, Brooklyn | Christiansburg | NT |