| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | FRIEND, Jackson | Christiansburg | 46.51 | Blue & Gold HS Invitational |
| 2 | GASTON, Tyler | Christiansburg | 42.11 | VHSL Class 3 State Championships |
| 3 | LODER, Joey | Glenvar High School | 38.85 | VHSL Region 2C Championships |
| 4 | CHEN, Nathan | Blacksburg High School | 41.92 | Golden Eagle Invitational |
| 5 | HENDRICKS, Jaden | Radford High School | 43.81 | Blacksburg Invitational |
| 6 | HITZLEBERGER, Kolbe | Blacksburg High School | 46.55 | Titan Track Classic |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | MONTGOMERY, Cowan | Christian Heritage Academy | 49.21 | Terrier Team Challenge |
| 3 | WONNER, Parker | Eastern Montgomery | 47.71 | VHSL 1C Regional Outdoor Champio |
| 4 | DEHART-LEWIS, William | Radford High School | 48.71 | Titan Track Classic |
| 5 | GALLIMORE, Peyton | Radford High School | 52.20 | Floyd MS - Three Rivers District |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HARRIS, Grayson | Glenvar High School | NT | |
| 3 | MACIE, Thierry | Christiansburg | NT | |
| 4 | DICKENS, Nathan | Glenvar High School | NT | |
| 5 | ANDERSON, Jack | Blacksburg High School | NT |