| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Chancellor | A | Setterlund | Kyere | Moody | Hill | NT |
| 2 | King George | B | Josephs | Ricardo | Bhatti | Downum | NT |
| 3 | James Monroe | A | Jackson | Jones | Conner | Swain | 51.90 |
| 4 | King George | A | Todd | Peters | Reynolds | Lane | 50.67 |
| 5 | Caroline High School | A | Baker | Veney | Jones | Coleman | 51.00 |
| 6 | Culpeper County | A | Claveloux | Claveloux | Norman | Dowd | 53.73 |
| 7 | Caroline High School | B | Few | Goodall | Ruffner | Smith | 55.00 |
| 8 | Courtland | A | NT |