| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | King George | B | Inzana | Tidwell | Lynch | Sabo | 4:00.00 |
| 2 | Chancellor | A | Betancourt | Green | Esa Kamara | Cole | 3:49.09 |
| 3 | Culpeper County | A | Brubaker | Johnson | Smith | Lane | 3:41.38 |
| 4 | Caroline High School | A | Berryman | Griffith | Morris | Terrell | 3:34.92 |
| 5 | Caroline High School | B | Lunn | Martin | Toliver | Duke | 3:39.92 |
| 6 | Spotsylvania | A | Booth | Miller-Jackson | Davidson | Jah | 3:48.09 |
| 7 | King George | A | Rash | Jones | Pelkey | Jones | 3:52.88 |