| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | ELBERT, Ann Louise | SO | Chicago (Lane) | 6:03.46 | |
| 2 | TRYFONOPOULOS, Viviana | FR | Chicago (Kennedy) | 6:07.00 | |
| 3 | BUTTS, Isabella (Ella) | SO | Chicago (Lane) | 6:15.00 | |
| 4 | LYNCH, Madeline | SO | Chicago (Lane) | 6:25.00 | |
| 5 | LAVERY, Ada | SO | Chicago (Lane) | 6:25.00 | |
| 6 | WILKEY, Margaux | FR | Chicago (Lane) | 6:25.00 | |
| 7 | RAMIREZ, Giselle | FR | Wheaton (North) | 6:28.80 | |
| 8 | HERNANDEZ-RUIZ, Katherine | SO | Chicago (Kennedy) | 6:49.71 | |
| 9 | AVILES, Eden | SO | Wheaton (North) | 6:57.65 | |
| 10 | STEVENS, Clara | SO | Wheaton (North) | 7:22.02 | |
| 11 | GONZALEZ, Evelyn | SO | Chicago (Kennedy) | 7:40.46 | |
| 12 | FORREST, Tawana | SO | Chicago (Kennedy) | 9:02.65 | |
| 13 | JOHNSTON, Emma | FR | Wheaton (North) | NT |