| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | HANLY, Molly | SO | Chicago (Lane) | 1:05.00 | |
| 2 | KARNEY, Kenna | JR | Wheaton (North) | NT | |
| 3 | MURPHY, Weller | JR | LaGrange Park (Nazareth Acad | NT | |
| 4 | HANSFIELD, Olivia | JR | Wheaton (North) | NT | |
| 5 | PEDERGNANA, Elizabeth | SO | LaGrange Park (Nazareth Acad | NT | |
| 6 | WHITE, Melody | SR | Hillside (Proviso West) | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | MALINOWSKI, Haydyn | JR | Wheaton (North) | NT | |
| 2 | RODRIGUEZ, Veda | JR | Chicago (Kennedy) | NT | |
| 3 | MCCAY, Kate | JR | Chicago (Lane) | 1:00.00 | |
| 4 | BAUTISTA, Anahi | SR | Chicago (Lane) | 1:00.00 | |
| 5 | AKBAR, Nyah | SR | Maywood (Proviso East) | NT | |
| 6 | CANO, Karen | JR | Chicago (Kennedy) | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | FAUL, Margaret | JR | Chicago (Lane) | 55.00 | |
| 2 | VERANGA, Sophia | JR | Chicago (Lane) | 49.00 | |
| 3 | NGUYEN, Emily | JR | Wheaton (North) | 48.87 | |
| 4 | DELLI BOVI, Cleo | SR | Chicago (Lane) | 49.00 | |
| 5 | GRUENWALD, Ava | SR | Wheaton (North) | 51.15 | |
| 6 | ANDES, Emily | JR | Chicago (Lane) | 58.00 |