| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Rogers Park Montessori | B | Lovesee | Adams | Shah | Smith | NT |
| 3 | Rogers Park Montessori | A | Desai | Gomez-Devia | Warnock | Smith | NT |
| 4 | Disney II | A | Schimmel | Prentice | Valdes | Bacchus | 1:07.84 |
| 5 | Disney II | B | Lorenzana | Sloan | Karsten | Vitayaudom | NT |
| 6 | St Matthias | A | Horan | Salib | Goddard | Kelly | 1:15.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Immaculate Conception North Park | A | Boryk | Angelo | Mueller | Blackburn | 1:08.70 |
| 2 | Immaculate Conception North Park | B | O'Hara | Love | Franky | Langer | NT |
| 3 | Lincoln (Chicago) | A | Reiffsteck | Zarei | Kooker | Shraiberg | 59.23 |
| 4 | Chicago Trailblazers | A | Shure | Buchar | Lee | Chapin | NT |
| 5 | Chicago Trailblazers | B | Massick | Lamb Jr. | Shutler | Milton | NT |