| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MORA, Francesca | 21-Unattached | 6:02.50 |
| 2 | GUTIERREZ, Isabella | MiniCRR | 6:26.76 |
| 3 | EGGERING, Isla | Chicago Trailblazers | 6:58.99 |
| 4 | MURCH, Olivia | Unattached | 7:00.00 |
| 5 | BILLENA, Keilee | Lincoln (Chicago) | 7:00.00 |
| 6 | MASSICK, Reese | Chicago Trailblazers | 7:00.00 |
| 7 | WILLIAMS, Addison | Catherine Cook | 7:05.10 |
| 8 | ZVEREVA, Polina | Lincoln (Chicago) | 7:17.42 |
| 9 | FERSCHINGER, Macy | Queen of Angels | 7:19.71 |
| 10 | O'HARA, Ellie | Immaculate Conception North Park | 7:41.50 |
| 11 | CASTELLI, Maren | St. Helen | NT |
| 12 | STUMP, Marlowe | Immaculate Conception North Park | NT |