| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 | Chicago (Noble/Rauner) | A | NT | |
| 2 | Chicago (Phillips) | A | 5:26.00 | |
| 3 | Chicago (Washington) | A | NT | |
| 4 | Chicago (Agricultural Science) | B | NT | |
| 5 | Chicago (Phillips) | B | NT | |
| 6 | Chicago (Noble/Butler) | A | NT | |
| 7 | Chicago (Noble/UIC) | A | NT | |
| 8 | Chicago (Agricultural Science) | A | NT | |
| 9 | Chicago (Juarez) | A | NT |