| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 3 | Chicago (Noble/UIC) | A | NT | |
| 4 | Chicago (Noble/Butler) | A | DQ | |
| 5 | Chicago (Curie) | A | NT | |
| 6 | Chicago (Noble/Butler) | B | NT | |
| 7 | Chicago (Noble/UIC) | B | NT | 
| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 | Chicago (Brooks) | C | NT | |
| 2 | Chicago (Hyde Park) | A | NT | |
| 3 | Chicago (Phillips) | B | NT | |
| 4 | Chicago (Phillips) | C | 4:00:00.00 | |
| 5 | Chicago (Phillips) | A | NT | |
| 6 | Chicago (Juarez) | A | NT | |
| 7 | Chicago (Instituto Health Science Charter) | A | NT | |
| 8 | Chicago (Brooks) | B | NT | 
| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 | Chicago (Simeon) | B | 52.00 | |
| 2 | Chicago (Corliss) | B | NT | |
| 3 | Chicago (Dyett) | B | 47.12 | |
| 4 | Chicago (Dyett) | A | 45.93 | |
| 5 | Chicago (Corliss) | A | 46.90 | |
| 6 | Chicago (Simeon) | A | 47.21 | |
| 7 | Chicago (Brooks) | A | 48.80 | |
| 8 | Chicago (Brooks) | D | 4:00.00 |