| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Prairie-Hills | A | Streeter | Johnson | Campbell | Mattison | NT |
| 2 | Jefferson (Champaign) | A | Mukoka | Jarrett | Butts-Towsend | Clark | DQ |
| 3 | Kankakee | A | Siller | Lee | White | Williams | 50.80 |
| 4 | Franklin (Champaign) | A | Pelmore | Davis | Jones | Gould | 52.61 |
| 5 | Bradley Central | A | Birk | Sherrod | Alejandro | Edmon | 52.86 |
| 6 | Washington (Chicago Heights) | A | Dawkins | East | Gray | Mckenzie | DQ |