| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Prairie-Hills | A | Murray | Riley | Hawkins | Williams | NT |
| 2 | Bradley Central | A | Perez | Lee | Tucker | Edmon | 53.12 |
| 3 | Franklin (Champaign) | A | Tinoco-Lewandowski | Dacanay | Deering | Allah | 52.61 |
| 4 | Kankakee | A | Turner | Andrade-Cintora | Turner | Johnson | DQ |
| 5 | Washington (Chicago Heights) | A | Darling | Nabors | Hernandez | Wade | NT |