| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Kroc Chicago Hounds & Foxes | A | Pleasant | Searcy | Shannon | Smith | NT |
| 3 | Frances Xavier Warde | A | Kennedy | Cox | Luu | Church | 2:32.98 |
| 4 | Old St Mary's Catholic Middle Sc | A | Waller | Pinchback | Cook | Sinkovich | 1:47.72 |
| 5 | Providence-Englewood Charter | A | Houston | Gray | Jordan | McClain | 2:16.21 |
| 6 | Clissold | A | Lyles | Fuentes | Turner | Smith | NT |
| 7 | St. Ethelreda | A | Aqeel | Carter | Aaron | Braylon | NT |