| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | St. Ethelreda | A | Makinley | White | Williamson | Crowder | NT |
| 4 | Frances Xavier Warde | A | Brevard | Ryerson | Zweidinger | Eldam | 2:19.27 |
| 5 | Catherine Cook School | A | Burns | Gauri | Linguanti | Rice | 2:41.42 |
| 6 | Providence-Englewood Charter | A | Bland | Mitchell | Nelson | Smith | NT |