| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SENSLEY, Thomas | UNAT-South Alabama | 50.07 |
| 2 | DAVIS, Keith | Unattached | 49.54 |
| 3 | PRICE, Derrick | William Carey |
48.99 |
| 4 | MADOURIE, Jovel | Unattached | 48.50 |
| 5 | BELL, Brandon | William Carey |
48.80 |
| 6 | PALMER, Shemar | William Carey |
49.31 |
| 7 | AUGUSTIN, Kristopher | William Carey |
49.56 |
| 8 | OWENS, Carey | Stillman |
50.18 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GAFFNEY, Joshua | Marion Military Institute | 53.47 |
| 2 | CURLEY, Quincy | Spring Hill |
53.18 |
| 3 | HOBSON, Jacob | Mobile |
52.24 |
| 4 | FRANKLIN, Kanye | West Alabama |
51.38 |
| 5 | WHITFIELD, Jourden | Miss. Univ. for Women | 51.78 |
| 6 | MACON, Tavonta | Miss. Univ. for Women | 52.55 |
| 7 | REID, Joevonie | Mobile |
53.24 |
| 8 | HALL-BAKER, KeYonn | Fontbonne |
54.01 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BRANDON, Corie | Rust |
NT |
| 2 | WILLIAMS, Eric | Spring Hill |
58.20 |
| 3 | RICHEY, Bradley | Marion Military Institute | 56.75 |
| 4 | DIXON, Donovan | West Alabama |
54.38 |
| 5 | SISK, Ethan | Spring Hill |
55.11 |
| 6 | BROWN, Brandon | Miss. Univ. for Women | 56.98 |
| 7 | DRUSKOFF, Tyler | Marion Military Institute | 1:01.54 |
| 8 | CLARK, Nathaniel | Rust |
NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | STREATE, Damonte' | Rust |
NT |
| 4 | EVANS, Xavier | Rust |
NT |
| 5 | PITTS, Alandon | Rust |
NT |