| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | PINES, Yasmin | Mobile |
1:02.99 |
| 2 | PRATT, Raven | Mobile |
1:01.79 |
| 3 | SHAMSID-DEEN, Sadiya | Mobile |
59.26 |
| 4 | MILES, Breanna | SE Missouri |
56.28 |
| 5 | HARDIN, Laila | SE Missouri |
56.58 |
| 6 | GUDDE, Tayler | SE Missouri |
1:00.88 |
| 7 | DWAAH, Leanza | SE Missouri |
1:02.56 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | AUBUCHON, Megan | SE Missouri |
NT |
| 4 | BROUSSARD, Ashlyn | Mobile |
1:03.55 |
| 5 | GORDON, Daja | SE Missouri |
1:05.90 |