| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | South Alabama |
A | Childress | Rogers | Stewart | Glen | 3:13.41 |
| 2 | South Alabama |
B | Collins | Glass | Reyneke | Shirley | 3:13.41 |
| 3 | Mobile |
C | Strachan | Wright | Sullivan | Morrison | DNS |
| 4 | Mobile |
B | Christopher | Evans III | Murphy | Richardson | 3:31.24 |
| 5 | Mobile |
A | Paquette | Reid | Hobson | Egalite | DNS |