| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MAGAUD, Adele | South Alabama |
4:42.56 |
| 2 | GIPE, Sidney | Mobile |
NT |
| 3 | BURGESS, Kayley | Mobile |
5:40.30 |
| 4 | JOHNSTON, Kayla | Mobile |
NT |
| 5 | PAGE, Trinity | Mobile |
NT |
| 6 | WEITENBECK, Abbey | South Alabama |
4:40.79 |
| 7 | TINKLER, Lauren | South Alabama |
NT |
| 8 | ACOSTA, Madisyn | Loyola-New Orleans |
NT |