| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Escambia | A | Steele | Washington | Mitchell | Dupree | 46.49 |
| 3 | Pensacola | A | Moody | Jackson | Bonner | Hughley | 45.45 |
| 4 | Washington (Pensacola) | A | Jenkins | Harris | Lewis | Stallworth | 43.14 |
| 5 | Pensacola Christian | A | Breitenstein | Cochran | Wilson | McKenzie | 45.33 |
| 6 | Pensacola Catholic | A | Nettles | Thompson | McGhee | Stackhouse | 46.36 |
| 7 | Northview | A | Hughes | White | Yoder | Weaver | 47.63 |