| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | CPU | A | Bark | Spore | Forman | Stowers | 48.31 | |
| 2 | Ottumwa | A | Derby | Brinegar | Barndt | Graeve | 46.86 | |
| 3 | Kennedy | A | Williams | Torgerson | White | Mann | 44.68 | |
| 4 | CR Prairie | A | Moeller | Rickertsen | Taylor | Lewis | 44.20 | |
| 5 | IC High | A | Rew | Harris | Major | Kueter | 44.50 | |
| 6 | CRX | A | Hartman | Hiserote | Mautino | Rexroth | FS | |
| 7 | IC Regina | A | Naeve | Meyer | Olney | Jensen | 47.25 |