| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clear Creek-Amana | A | Patrick | Overman | Burken | Duncan | 2:12.00 | |
| 3 | Williamsburg | A | Stratton | Folkmann | Stohlmann | Hirko | 2:01.00 | |
| 4 | Grinnell | A | Ostby | Vanderleest | Corey | Gallagher | 1:58.11 | |
| 5 | Sigourney | A | Wilson | Crow | Reed | Wehr | 2:01.00 | |
| 6 | Vinton-Shellsburg | A | Barnes | McClintock | Osborn | Dickenson | 2:10.00 | |
| 7 | CCA- White | A | Michel | Colvin | Strank | Trana | 2:13.00 |