| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Grinnell | A | Kelso | Sieck | Thompson | Rhinehart | 4:58.08 | |
| 3 | Clear Creek-Amana | A | Halversen | Meskimen | Umoren | Sorensen | 4:40.00 | |
| 4 | Williamsburg | A | Sinn | Reese | Heitman | Hocker | 4:13.08 | |
| 5 | Sigourney | A | Bruns | Clark | Wehr | Klett | 4:25.00 | |
| 6 | CCA- White | A | Frank | Moquin | Haughy | Hilsman | 4:50.00 |