| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | CCA- White | A | Michel | Trana | Strank | Waring | 6:00.00 | |
| 3 | Grinnell | A | Eisen | Corey | Ostby | Freeman | 4:40.96 | |
| 4 | Vinton-Shellsburg | A | Fisher | Johnson | Blais | Torres | 4:36.00 | |
| 5 | Williamsburg | A | Stratton | Stohlmann | Simmons | Brummel | 4:40.00 | |
| 6 | Clear Creek-Amana | A | Bonde | Overman | Burken | Vazquez | 5:00.00 |