| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clear Creek-Amana | A | Brandt | Romero | Leighton-Dick | Stopko | 2:25.00 | |
| 3 | Williamsburg | A | Simmons | Winegarden | Wetjen | Rich | 2:21.00 | |
| 4 | Mount Vernon | A | Meester | Jackson | Schoff | Moss | 2:11.00 | |
| 5 | Grinnell | A | Harms | Neighbors | Lavender | Kiphanzu | 2:18.32 | |
| 6 | Benton | A | Lennie | Reynolds | Schroeder | Drahos | 2:22.49 | |
| 7 | Solon | A | Sell | Billerbeck | Miller | Hendricks | 4:55.29 |