| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cornell College |
A | Engelken | O'Hara | Watters | Cowan | DNS | |
| 2 | Viterbo |
A | Savage | Jenkins | Lindstrom | Anderson | 44.99 | NSAA Outdoor Championships |
| 3 | Mount Mercy |
A | Powell | Christophersen | Bell | Harmon | 44.50 | |
| 4 | St. Ambrose |
B | Zachary | Rush | Polk | Davis | DNS | |
| 5 | Hawkeye CC |
A | Elliott | Tapia | Kajtezovic | Seth | 44.00 | |
| 6 | Dubuque |
C | Hardison | Byrd | Spires | Gabrys | 44.51 | |
| 7 | Dubuque |
D | Anderson | Alshomrani | Sigwarth | Atwater | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Dubuque |
B | Santiago | Wierick | Gohde | Griffin | 43.95 | |
| 2 | North Iowa Area CC |
A | Jackson | Jackson | McNight | Lee-Green | 43.00 | |
| 3 | Iowa Central CC |
B | Brown | Hicks-Jumper | Scott | Wilson | 42.50 | |
| 4 | Iowa Central CC |
A | Jessup | Hayles | Eppie | Dyer | 40.39 | USD Early Bird |
| 5 | Dubuque |
A | Lentz | Oldham | Williams | Atwater | 42.37 | |
| 6 | St. Ambrose |
A | Olenek | Reemtsma | Reynolds | Reemtsma | 42.98 | |
| 7 | Waldorf |
A | Lopes | Thomas | Minor | Barrera | 43.50 | |
| 8 | Coe |
A | Weber | Rigdon | Fleck | Cory | 44.00 |