| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | JONES, David | FR | Clarke |
57.82 | 2021 Viking Classic |
| 3 | WILKERSON, Keyondrae | FR | Coe |
56.00 | |
| 4 | HUNT, Spencer | SO | Buena Vista |
54.58 | Dennis Young Classic |
| 5 | ARCENEAUX, Sabian | FR | Coe |
56.00 | |
| 6 | POWERS, Max | JR | Buena Vista |
57.55 | 57.55 Dennis Young Classic |
| 7 | MILLER, Kyle | FR | Buena Vista |
NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MURPHY, Max | SO | Buena Vista |
54.52 | |
| 2 | LINK, Chis | FR | Sauk Valley CC |
53.09 | 2021 Green & White Invite |
| 3 | MICKE, Jadon | SO | Buena Vista |
51.05 | American Rivers Indoor Conference Championships |
| 4 | SCHROEDER, Jordan | FR | Mount Mercy |
50.89 | |
| 5 | QUINONEZ, Gyvanziel | FR | Mount Mercy |
50.99 | |
| 6 | SYLVESTER, Jackson | SO | Buena Vista |
51.62 | 2021 Division III Elite Indoor Championships |
| 7 | BAIRD, Anthony | JR | Buena Vista |
54.14 | 2021 Kip Janvrin Open |
| 8 | WELLS, Draven | FR | Sauk Valley CC |
54.54 | Mustang Open |