| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Center Point-Urbana | A | Hansen | Kreel | Hunt | Ziltergruen | 55.10 | |
| 3 | Solon | A | Barnhouse | Levin | Miller | Bachus | 53.00 | |
| 4 | Lisbon | A | Happel | Czarnecki | Schnipkoweit | Robinson | 52.57 | |
| 5 | Marion | A | Burlage | Johnson | Meth | Poston | 52.93 | |
| 6 | Benton Community | A | Lamer | Vollbrecht | Townsley | Sims | 54.44 |