| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | East Bay |
A | McCurry | Dixon | McCurry | Martinez | 3:43.29 | Riverview Shark Frenzy |
| 4 | Riverview |
A | West | Strickland | Persaud | Blackmon III | 3:37.46 | Jack Rice Invitational 2026 |
| 5 | Spoto |
A | Replogle | Jones | Harris | Riggins | 3:39.16 | 2026 Manuel Griffin Fraundorfer Relays |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Morgan High School | A | Hall | Powell | Perry | Cook | 3:32.73 | Riverview Shark Frenzy |
| 3 | Lennard |
A | Merritt | Williams | Brooks | Gibbons | 3:28.35 | Lennard 5 Way Meet |
| 4 | Newsome |
A | Johnston | Campbell | Easley | O'Bryant | 3:20.99 | 2026 Manuel Griffin Fraundorfer Relays |
| 5 | Bloomingdale |
A | Smith | Gonzalez | Lowe | Chestnut | 3:27.81 | 2026 Manuel Griffin Fraundorfer Relays |
| 6 | Sumner |
A | Botardo | Henry | Freeman | Haugabook | 3:29.89 | FSU Relays (High Schools) 2026 |
| 7 | Plant City |
A | Urena | Horton | Cipriano | Steed | 3:35.33 | Bob English Invitational 2026 |