| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ROBERTS, Amaizen | Spoto |
NT | |
| 2 | CARR, Kayla | Strawberry Crest |
33.20 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 3 | DAWSON, Aria | Newsome |
29.54 | Durant Senior Night 2026 |
| 4 | RUDDOCK, Madison | Newsome |
29.07 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 5 | BROWNE, Aysia | Newsome |
29.26 | Spoto Senior Night 2025 |
| 6 | PEREZ, Aryanna | Strawberry Crest |
33.10 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 7 | ERDAIN, Abigaelle | Strawberry Crest |
NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JOSEPH, Nora | Spoto |
28.57 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 2 | DAWSON, Aleia | Newsome |
27.33 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 3 | JOHNSON, Brielle | Spoto |
26.20 | FHSAA 3A Region 3 |
| 4 | CULPEPPER, Cai | Tampa Prep |
23.69 | FHSAA State Track & Field Championships (1A) |
| 5 | CULPEPPER, Corrie | Tampa Prep |
25.73 | Tampa Jesuit Meet 1 |
| 6 | GILYARD, Sky | Tampa Prep |
27.00 | Tampa Jesuit Meet 1 |
| 7 | FRAZIER, Alivia | Tampa Prep |
27.38 | Jesuit Meet #5 |
| 8 | SNEAD, Maleah | Spoto |
28.80 | Middleton Tiger Senior Night 2025 |