| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | BROWN, Savannah | Newsome |
21.49 | 2026 TRC King Invitational |
| 5 | BERREBI, Marlee | Newsome |
18.94 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 6 | BACON, Lela | Newsome |
20.76 | Durant Senior Night 2026 |
| 7 | CINTRON, Izabella | Strawberry Crest |
22.12 | Jack Rice Invitational |