| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Morgan High School | A | Payton | Teartt | Brown | Carter | NT | |
| 4 | Spoto |
B | Currington | Nuriddin | Lopez | Johnson | 1:00.00 | |
| 5 | Morgan High School | B | Chestnut | Downes | Ratcliff | Mchayle | DQ |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Spoto |
A | Joseph | Roberts | Hatter | Snead | 59.00 | |
| 2 | Riverview |
A | Fanchi | Lee-Shung | Garlic | Campbell | 48.85 | 2025 Nash Higgins Relays |
| 3 | Newsome |
A | Branche | Dawson | De Souza | Ramos | 48.00 | FHSAA 4A Region 3 |
| 4 | Lennard |
A | Rowells | Walters | Johnson | Easy | 47.61 | FHSAA 4A Region 3 |
| 5 | Newsome |
B | Alaalaoui | Dawson | Browne | Daniels | 48.00 | FHSAA 4A Region 3 |
| 6 | Riverview |
B | Allen | Watts | Crossman | McCormick | 48.85 | 2025 Nash Higgins Relays |
| 7 | Strawberry Crest |
A | Starford | Georges | Carr | Perez | DNS | FHSAA 4A District 9 |