| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bloomingdale |
A | Staley | Millet | Melendez | Vivanco | 8:13.10 | 2025 Vernon Korhn Hillsborough County Championships |
| 2 | Sumner |
A | Beniquez | Henry | Freeman | Phelps | 8:23.52 | FSU Relays (High Schools) |
| 3 | Riverview |
A | Cardin | Capell | Choma | Makinde | 8:38.12 | FHSAA 4A Region 3 |
| 4 | East Bay |
A | McCurry | MGarrah | Dalmida | Williams | 9:13.72 | Jack Rice Invitational |
| 5 | Lennard |
A | Jorden | Jorden | Salgado | Leon | 9:22.70 | 2025 Lennard Senior Night |
| 6 | Brandon |
A | Brown | Del Real | Parise | Singleton | 10:17.11 | 2026 Manuel Griffin Fraundorfer Relays |