| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Morgan High School | A | Huggins | Gachette | Brown | Royal | DNS | 2026 Nash Higgins Relays |
| 3 | Riverview |
A | Davis | Lowe | Badillo Perez | Holdip | 4:16.04 | 2026 Manuel Griffin Fraundorfer Relays |
| 4 | Lennard |
A | Peterson | Callaway | Sampson | Reynoso | 4:01.82 | FHSAA 4A Region 3 |
| 5 | Bloomingdale |
A | Hayes | Courtney | Falcon | Golden | 4:08.88 | FHSAA 4A Region 3 |
| 6 | East Bay |
A | Sherman | Smith | Smith | Marshall | DNS | FHSAA 3A Region 3 |