| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Coloma | A | Rogers | Price | Athan | Hutchins | 1:01.54 |
| 2 | Berrien County Youth Track | A | Tomsa | Tomsa | Chandler | Malaka | 1:00.00 |
| 3 | Kalamazoo Christian | A | Boley | Bos | Page | Tabb | 56.81 |
| 4 | Hartford | A | Johnson | Hartline | Ledesma | Garcia | 58.85 |
| 5 | Bridgman | A | Robinson | Thomas | Nelson | Ullig | 1:01.33 |
| 6 | Kalamazoo Homeschool Sports | A | DeYoung | Babb | Hanoute | Bowman | 1:03.71 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Constantine | A | Bolle | Cullifer | Avila | Walker | 55.65 |
| 2 | Benton Harbor | A | Page | McCalvin | Anderson | Evans | 53.40 |
| 3 | Niles Brandywine | A | Price | Byrd | Price | Colbert-Lorenz | 52.95 |
| 4 | Dowagiac Union | A | Clark | Mack | Richter | Poorman | 53.22 |
| 5 | St Joseph Michigan Lutheran | A | Fletcher | Suggs | Huff | Liss | 54.64 |
| 6 | Hopkins | A | Bonga | Benting | EspaƱa-Morillo | Runkel | 55.86 |