| PL | Athlete | Team | Time | Wind | Pts | HPL | 
|---|---|---|---|---|---|---|
| 1 | CUNNINGHAM, Kamille | 36 Elite MOVES | 26.11 | -1.0 | 20 | 2 (1) | 
| 2 | GRIFFIN, Jalencia | SC Express Track Club | 26.25 | -1.0 | 16 | 2 (2) | 
| 3 | OLOPADE-SIMMONS, Aliyah | The E.L.I.T.E.S. | 26.33 | -1.0 | 12 | 2 (3) | 
| 4 | THOMPSON, Bryanna | FAST FORWARD TRACK CLUB | 26.88 | -1.0 | 10 | 2 (4) | 
| 5 | GIBSON, Madison | SC Express Track Club | 27.16 | -1.0 | 8 | 2 (5) | 
| 6 | PATRICK, Evelyn | The Storm Track Club | 27.39 | -1.5 | 6 | 1 (1) | 
| 7 | SINGLETARY, Nya | FAST FORWARD TRACK CLUB | 27.46 | -1.5 | 4 | 1 (2) | 
| 8 | JONES, Phiasia | FAST FORWARD TRACK CLUB | 27.69 | -1.5 | 2 | 1 (3) | 
| 9 | COOK, Angel | The Storm Track Club | 27.87 | -1.0 | 2 (6) | |
| 10 | EUGENE, Faith | Extra Mile Tigers | 28.38 | -1.5 | 1 (4) | |
| 11 | WELLS, Taylor | Dreamchaserz | 29.06 | -1.5 | 1 (5) | |
| 12 | NIMO, Karen | ASAP Performance | 29.39 | -1.0 | 2 (7) | |
| 13 | CANTY, Za'Niaha | Columbia Knights | 30.64 | -1.0 | 2 (8) | |
| 14 | HICKS, Sheriyah | Bionic Sports Performance | 32.79 | -1.5 | 1 (6) |