| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Union Athletic Association | B | Roden | Lockrem | Bryan | Benoit | NT | |
| 3 | City of Monroe | A | Rideout | Colston | Watkins | Ware | 1:04.50 | |
| 4 | James Recreational Camps- JRC | A | Patterson | Smith | Maness | Wentz | 57.56 | |
| 5 | Union Athletic Association | A | Sheriffe | Hammonds | Stevens | Stevens | 1:02.24 | |
| 6 | City of Monroe | B | Marsh | Smith | Exantus | Whittley | NT |