| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | City of Monroe | C | Nelson | Wolf | Smith | Estep | NT | |
| 3 | City of Monroe | A | Casey | Latham | Russ | Artis | 1:04.50 | |
| 4 | James Recreational Camps- JRC | A | McMullen | Jennings | Jones | Scott | 57.56 | |
| 5 | Union Athletic Association | A | Davis | Morrow | Morrow | Missick | 1:02.24 | |
| 6 | City of Monroe | B | Burke | McManus | Marsh | Stevens | NT |