| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | City of Monroe | D | Williams | Wolf | Wolf | Holloway | NT | |
| 3 | City of Monroe | C | Christian | Wilson | Shipley | Lewis | NT | |
| 4 | James Recreational Camps- JRC | A | Gomez-Enriquez | Tyson | Valle | Chambers | DNF | |
| 5 | City of Monroe | A | Artis | Clyburn | Richardson | Rideout | 1:05.86 | |
| 6 | City of Monroe | B | Reid | Porter | Merriman | Womack | NT | |
| 7 | James Recreational Camps- JRC | B | Gomez-Enriquez | Delao | Rivera | Montgomery | NT |