| Lane | # | Athlete | Team |
|---|---|---|---|
| 1 | 859 | HUTTON, Eliza | Fletcher |
| 2 | 197 | NICHOLS, Mikaela | Fernandina Beach |
| 3 | 809 | SHARP, Addison | Wolfson |
| 4 | 879 | MURILLO, Clara | Fletcher |
| 5 | 348 | MUNN, Monica | Mandarin |
| 6 | 499 | MIRIJANYAN, Yeva | Atlantic Coast |
| 7 | 766 | MYERS, Emily | Wolfson |
| 8 | 243 | OSTOVAR, Samantha | Gainesville |
| 9 | 742 | WAGNER, Grace | Atlantic Coast |
| 10 | 495 | KING, Siena | Atlantic Coast |
| 11 | 358 | JOHNSON, Kacee | Mandarin |
| 12 | 44 | BOLES, Jayden | West Port |
| 13 | 339 | HANDMAKER, Sadie | Mandarin |
| 14 | 136 | GUTIERREZ-JIMENEZ, Jackeline | Riverside |
| 15 | 931 | RILEY, Elliott | Fletcher |
| 16 | 120 | WALKER, Taylor | Riverside |
| 17 | 177 | WASHINGTON, Shaniya | Riverside |
| 18 | 309 | VIALIZ, Brianna | Mandarin |
| 19 | 284 | RANT, Elizabeth | Gainesville |
| 20 | 753 | SANTANA-TIRADO, Amanda | Global Academy |
| 21 | 26 | ESPINZOA, Brianna | West Port |
| 22 | 239 | HOWARD, Jade | Gainesville |
| 23 | 328 | BOTTA-WATSON, Krishae | Mandarin |
| 24 | 342 | MUNN, Marlee | Mandarin |