| Lane | # | Athlete | Team |
|---|---|---|---|
| 1 | 923 | STREETER, Cameron | Fletcher |
| 2 | 126 | LEWIS, Devon | Riverside |
| 3 | 79 | CONEY, Arion | West Port |
| 4 | 878 | CAFE, Quinn | Fletcher |
| 5 | 92 | CLEMENTE JR., Anthony | West Port |
| 6 | 263 | CHATMAN, Keishaun | Gainesville |
| 7 | 831 | HOLMES, Grant | Wolfson |
| 8 | 106 | TELISMAR-WILLIAMS, D'Andre | West Port |
| 9 | 702 | NATTIEL, Khayari | Newberry |
| 10 | 757 | BEVINS, Jaden | Global Academy |
| 11 | 749 | AMBROSIANI, Christian | Global Academy |
| 12 | 692 | DAVIS, Lamarkeise | Newberry |
| 13 | 346 | BRYANT, Trinton | Mandarin |
| 14 | 856 | HARRIS, Kemarley | Fletcher |
| 15 | 940 | THOMAS, Jalani | Fletcher |
| 16 | 706 | KING, Lewis | Newberry |
| 17 | 222 | WILLIAMS, Derek | Gainesville |
| 18 | 357 | IBRAHIMBEGOVIC, Emin | Mandarin |
| 19 | 839 | CACCUM, Isaac | Wolfson |
| 20 | 647 | WOLESHIN, Gavin | Fernandina Beach |
| 21 | 664 | JORDAN, Jerimiah | Fernandina Beach |
| 22 | 308 | GLIN, Kurtis | Mandarin |
| 23 | 154 | HILLIARD, Amare | Riverside |
| 24 | 603 | PECHA, Gunnar | Fernandina Beach |
| 25 | 211 | LEWIS-THOMPSON, Izaiah | Gainesville |