| Lane | # | Athlete | Team |
|---|---|---|---|
| 1 | 33 | KING, Tahlya | Andrew Jackson |
| 2 | 392 | WIGHT, Elle | Stanton |
| 3 | 204 | MURILLO, Clara | Fletcher |
| 4 | 1087 | MCCARDELL, Mylah | Yulee |
| 5 | 255 | VALENCIA, Julianna Marina | Oakleaf |
| 6 | 290 | SINGH, Gurbani | Paxon |
| 7 | 384 | PARMAN, Audrey | Stanton |
| 8 | 390 | TOMSON, Samantha | Stanton |
| 9 | 108 | YOACHUM, Rylie | Baldwin |
| 10 | 346 | HOPKINS, Lena | Sandalwood |
| 11 | 348 | LEE, Amber | Sandalwood |
| 12 | 201 | MARINO, Gwenyth | Fletcher |
| 13 | 1093 | ROBLEDO, Chloe | Yulee |
| 14 | 163 | MYERS, Eri'annya | First Coast |
| 15 | 198 | GALLAGHER, Alana | Fletcher |
| 16 | 354 | SHORT, Brianna | Sandalwood |
| 17 | 87 | WATLEY, India | Atlantic Coast |
| 18 | 73 | ABEL KHALEK, Aline | Atlantic Coast |
| 19 | 1028 | HAMPTON, Ahshaylyn | Raines |
| 20 | 156 | HARRELL, Emani | First Coast |
| 21 | 75 | CANTERBURY, Alyssa | Atlantic Coast |