| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WILLIAMS, Khylee | Eagle Athletics | NT |
| 2 | LAFSER, Harper | No Excuses RC | NT |
| 3 | MALLARY, Janiya | Eagle Athletics | 1:30.00 |
| 4 | BRIGGS, Zoei | Eagle Athletics | 1:20.00 |
| 5 | AJAGBE, Ava | Eagle Athletics | 1:29.00 |
| 6 | FIELDS, Joi | Tallahassee Zoom Track Club | NT |
| 7 | SMITH, Ari | 40s Showcase Entertainment | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | AJAGBE, Mia | Eagle Athletics | NT |
| 4 | HARTLEY, Vivian | No Excuses RC | NT |
| 5 | DANIELS, Zemira | Eagle Athletics | NT |