| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | River City Science Innovation | A | 54.17 |
| 2 | IDEA Hope College Preparatory MS | A | 52.21 |
| 3 | Bradford Middle School | A | 49.92 |
| 4 | University Christian School | A | 44.25 |
| 5 | Fernandina Beach Middle School | A | 48.77 |
| 6 | Bradford Middle School | B | 51.00 |
| 7 | KIPP Voice Academy | A | 53.59 |
| 8 | Duval Charter School at Flagler | A | 1:02.04 |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 2 | Tallahassee Zoom Track Club | A | NT |
| 3 | KIPP Impact Middle School | A | NT |
| 4 | Duval Charter School at Flagler | B | 1:02.04 |
| 5 | KIPP BCA | A | NT |
| 6 | KIPP BCA | B | NT |