| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SPARKMAN, Finley | Creeks Track & Field | 43.90 |
| 2 | NEVOLIS, Avery | Creeks Track & Field | 42.43 |
| 3 | DRAGICH, Scarlett | Creeks Track & Field | 40.29 |
| 4 | PEREZ, Selina | Creeks Track & Field | 42.11 |
| 5 | HENSLEY, Arabella | Creeks Track & Field | 43.18 |
| 6 | BEIDELMAN, Gabriella | Creeks Track & Field | 45.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | FRICKE, Pyper | Creeks Track & Field | 50.50 |
| 3 | MAZZO, Skye | Creeks Track & Field | 45.45 |
| 4 | MAZZO, Oceania | Creeks Track & Field | 45.80 |