| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | HILL, Kennedy | Andrew Jackson |
2:15.85 |
| 2 | BARRINGTON, Samari | Andrew Jackson |
2:28.00 |
| 3 | MIDDLETON, Jehzaria | Raines |
2:30.72 |
| 4 | PAIGE, Janaya | First Coast |
2:38.03 |
| 5 | GARNES, Nijah | Paxon |
2:42.75 |
| 6 | HARRIS, Jamiah | First Coast |
2:52.88 |
| 7 | MIRIJANYAN, Yeva | Atlantic Coast |
2:53.39 |
| 8 | BARNES, Maddison | West Nassau |
2:54.27 |
| 9 | SHUFORD, Mariah | Riverside |
2:55.31 |
| 10 | GANCEREZ, Brianna | West Nassau |
2:56.11 |
| 11 | SINGH, Gurbani | Paxon |
2:56.32 |
| 12 | PASKE, Hanna | West Nassau |
2:56.90 |
| 13 | GUTIERREZ-JIMENEZ, Jackeline | Riverside |
3:00.81 |
| 14 | HARDIMAN, Arianna | Andrew Jackson |
3:02.69 |
| 15 | ORRETT, Kristen | Terry Parker |
3:05.59 |
| 16 | LOUIMA, Nyesha | Terry Parker |
3:15.05 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BUNCH, Iseballa | Bradford |
3:15.27 |
| 2 | TEK-DIXON, Delilah | Terry Parker |
3:32.48 |
| 3 | MEZA, Dulce | Charlton County | NT |
| 4 | MARSEILLE, Unique | First Coast |
NT |
| 5 | KAY-WRIGHT, Aleelah | Westside |
NT |
| 6 | BARTLEY, Taniyah | Andrew Jackson |
NT |
| 7 | SANTOS, Sophie | West Nassau |
NT |
| 8 | SMOTHERS, Maleah | Raines |
NT |
| 9 | PHILYAW, Jhinema | Riverside |
NT |
| 10 | MEZA, Anahi | Charlton County | NT |
| 11 | SLACK, Jenna | Atlantic Coast |
NT |
| 12 | WOODEN, Reignah | Baldwin |
NT |
| 13 | RIDLEY, Khyona | Ribault |
NT |
| 14 | KING, Siena | Atlantic Coast |
NT |
| 15 | RAWLS, Morgan | Atlantic Coast |
NT |