| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 |
|---|---|---|---|---|---|---|
| 1 | Florida Deaf and Blind | B | Essex | Essex | Garton | Lopez Martinez |
| 2 | Florida Deaf and Blind | A | Allen | Zahira | Torres | Vazquez |
| 3 | St. Joseph | A | Evans | Bateh | Castro | Hemelt |
| 4 | Ridgeview (OP) | A | Brown | Willis | Matz | Roguski |
| 5 | St. Augustine | A | Gribble | Lomaglio | Spindle | Allen |
| 6 | St. Augustine | B | Cernese | Dougherty | Washburn | Swesey |
| 7 | Global Outreach Charter | A | Ambrosiani | Rivera | Fuentes-Ledee | Sanchez |
| 8 | Ridgeview (OP) | B | Zamor | Light | Evans | Monroe |