| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | DAWKINS, London | River City FINISH | 1:01.00 |
| 2 | THOMPSON, Tailin | Unattached | 58.55 |
| 3 | JHAGROO, Sanau | Take Flight Athletics | 56.50 |
| 4 | SINGLETON, Nia | Take Flight Athletics | 55.80 |
| 5 | ST. JULIAN, Bree | Velocity Track Club | 55.91 |
| 6 | SARRATT, Alinah | Track Legend AC | 58.00 |
| 7 | WALKER, Annabel | Track Legend AC | 1:00.00 |
| 8 | HARRELL, Monica | Raines | 1:03.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MAY, Elizabeth | Unattached | 1:19.00 |
| 2 | MCDERMOTT, Adrienne | Elite Track and Field | 1:07.00 |
| 3 | MARTINEZ, Amya | River City FINISH | 1:05.00 |
| 4 | HUPP, Mary | JAC | NT |
| 5 | WALKER, Zoe | JAC | NT |
| 6 | MULBAH, Emani | Unattached | 1:07.00 |
| 7 | BAKER, Kazzaryia | Take Flight Athletics | 1:08.00 |
| 8 | FELICIANO, Barbara | River City FINISH | NT |