| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SINGLETON, Nia | Take Flight Athletics | 25.30 |
| 2 | JACKSON, Shyann | Unattached | 24.52 |
| 3 | ST. JULIAN, Elan | Velocity Track Club | 24.39 |
| 4 | PETERS, Nicola | HAMteam Huntsville Elite | 25.00 |
| 5 | MCMILLEN, Heather | HAMteam Huntsville Elite | 24.61 |
| 6 | PARKER, Zaria | HAMteam Huntsville Elite | 24.94 |
| 7 | HARRIS, Ashanetta | Unattached | 25.11 |
| 8 | ST. JULIAN, Bree | Velocity Track Club | 25.50 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ALICEA, Avery | Unattached | 26.56 |
| 2 | CLARK, Leigha | Take Flight Athletics | 26.10 |
| 3 | WILLIAMS-GOINS, Jaida | Take Flight Athletics | 25.80 |
| 4 | BING, Kalia | River City FINISH | 25.50 |
| 5 | JHAGROO, Sanau | Take Flight Athletics | 25.50 |
| 6 | JONES, Genesis | JD Athletics | 26.00 |
| 7 | STEPHENS, Anna | HAMteam Huntsville Elite | 26.50 |
| 8 | THOMPSON, Tailin | Unattached | 26.59 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | KLEIN, Ava | JAC | 28.40 |
| 2 | JENKINS, Trinity | Take Flight Athletics | 28.20 |
| 3 | O'CONNOR, Olivia | HAMteam Huntsville Elite | 28.00 |
| 4 | DAWKINS, London | River City FINISH | 26.75 |
| 5 | EVANS, Anijha | HAMteam Huntsville Elite | 27.00 |
| 6 | WHITE, Natalie | JD Athletics | 28.00 |
| 7 | BAKER, Kazzaryia | Take Flight Athletics | 28.30 |
| 8 | DAVIS, Ava | JAC | 28.50 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | OXENDINE, Iyana | Menendez | NT |
| 2 | MAY, Elizabeth | Unattached | 36.00 |
| 3 | CLAYTOR, Zoe | JAC | 28.90 |
| 4 | MCDERMOTT, Adrienne | Elite Track and Field | 28.60 |
| 5 | HARRELL, Monica | Raines | 28.74 |
| 6 | HUPP, Mary | JAC | 28.90 |
| 7 | CRUZ, Jarelys | River City FINISH | NT |
| 8 | MOHAMMED, Brianna | JAC | NT |