| Lane | Team | Squad | Seed |
|---|---|---|---|
| 2 | Duval Charter Baymeadows | A | 45.89 |
| 3 | Menendez | A | 45.00 |
| 4 | Oakleaf | A | 42.31 |
| 5 | Tocoi Creek | A | 43.45 |
| 6 | St. Joseph | A | 45.77 |
| 7 | Florida Deaf and Blind | A | 46.13 |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 3 | Global Outreach Charter | A | NT |
| 4 | KIPP Bold City High School | A | 48.55 |
| 5 | Florida Prep | A | NT |